Molecular and restriction fragment length polymorphism analysis of canine parvovirus 2 (CPV-2) in dogs in southeast Anatolia, Turkey

Molecular and restriction fragment length polymorphism analysis of canine parvovirus 2 (CPV-2) in dogs in southeast Anatolia, Turkey

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Canine parvovirus-2 (CPV-2) is the aetiological agent of an infectious viral disease of dogs, characterised by diarrhoea and vomiting.Mutations of the CPV-2 genome have generated new variants circulating worldwide.This article reports the molecular analysis of CPV-2 variants collected in the dog population in southeast Anatolia, Turkey.Twenty blood samples previously taken arcade smokey the bear belt for the laboratory diagnosis of dogs with suspected parvovirus were screened for CPV-2 by polymerase chain reaction (PCR).Of the 20 samples, 18 tested positive for CPV-2.

Partial VP2 gene sequencing and restriction fragment length polymorphism (RFLP) analysis revealed CPV-2a (n = 1), CPV-2b (n = 16) and CPV-2c (n = 1) variants.Phylogenetic analysis based on the partial length VP2 gene showed that CPV-2b (n = 15) sofia barclay sexy variants showed sequences clustering separately in the phylogenetic tree.The CPV-2c sample was phylogenetically related to Chinese strains and Indonesia strain, whereas the CPV-2a sample was phylogenetically related to the Portuguese strain.These results, which are the first to demonstrate the presence of CPV-2c in the dog population of southeast Anatolia, Turkey, indicate that CPV-2a/2b/2c variants co-exist in Turkey’s dog population.